●問題1

貨號(hào)：A137

品名：Factor H

品牌：complementtech

原文：

Q:May I ask if different batches of this product (A137) can be mixed and if this antibody has been tested for endotoxins?

A:Sorry for the delay.

Yes, you can mix the lots.  You will have to test, etc for activity.  If endotoxin tests were performed it would be listed on the applicable CoA.  If it is not listed then we did not test for endotoxins.

譯文：

問：請(qǐng)問該產(chǎn)品（A137）的不同批次能否混合使用？該抗體是否做過內(nèi)毒素檢測(cè)？

答：抱歉回復(fù)延遲。

不同批次的產(chǎn)品可以混合使用，你需要對(duì)混合后的產(chǎn)品進(jìn)行活性等相關(guān)檢測(cè)。如果產(chǎn)品做過內(nèi)毒素檢測(cè)，相關(guān)結(jié)果會(huì)在對(duì)應(yīng)的分析報(bào)告中列出；若報(bào)告中未提及，則說明該產(chǎn)品未進(jìn)行內(nèi)毒素檢測(cè)。

●問題2

貨號(hào)：10-0027-1

品名：Protein L Ligand Leakage ELISA

品牌：Medicago

原文：

Q:Customer feedback: Why has the expiration date of protein L reference （lot：285004） from the same batch been extended from 2025-10 to 2026-8 and 2027-8? Have you retested the protein L reference? If so, could you provide relevant statements and data?

A:Yes, we have extended the expiration date for the entire kit, including the references. There is no better proof than these certificates that the references have the same expiration date as the kit.

譯文：

問：客戶反饋：同批次的蛋白L標(biāo)準(zhǔn)品（批號(hào)：285004）有效期為何從2025年10月分別延長至2026年8月和2027年8月？貴司是否對(duì)該蛋白L標(biāo)準(zhǔn)品重新進(jìn)行了檢測(cè)？若有，煩請(qǐng)?zhí)峁┫嚓P(guān)說明及檢測(cè)數(shù)據(jù)。

答：我們已延長了整套試劑盒（包括參考品在內(nèi)）的有效期。這些證書就是參考品與試劑盒有效期一致的最有力證明。

●問題3

貨號(hào)：M050-1

品名：GPIbbeta (CD42c)Xia.C3 Rat IgG2a FITC; 300 tests

品牌：emfret

原文：

Q:The customer would like to inquire about the concentration of this antibody (M050-1)

A:Thank you for your inquiry.

We titrate the fluorescence-conjugated antibodies in relation to our negative controls (P190-X) so that optimal results are achieved. The ab concentration is not determined afterwards.

I am sorry to have no other information.

譯文：

問：客戶想咨詢?cè)摽贵w（M050-1）的濃度是多少？

答：感謝你的咨詢。

我們以P190-X陰性對(duì)照為參照，對(duì)熒光標(biāo)記抗體進(jìn)行滴定，以確保實(shí)驗(yàn)?zāi)艿玫阶罴呀Y(jié)果，后續(xù)并未測(cè)定該抗體的濃度。

很抱歉，目前沒有更多相關(guān)信息可以提供。

●問題4

貨號(hào)：A137

品名：Factor H

品牌：complementtech

原文：

Q:Hello, may I ask how the extinction coefficient of A137 was obtained? Was it measured experimentally or by other means? It differs quite significantly from the theoretical extinction coefficient based on the sequence. Thank you.

A:Hi Jane,

We have determined FH concentration spectrophotometrically using an extinction coefficient A280 nm (1% solution) of 12.4 (Ref#1). Dr. MK. Pangburn has extensively worked on Factor H. Based on the explanation given by Dr. MK Pangburn in his paper here is the summary: Extinction coefficients reported for factor H have been reported ranging from 9.0 to 19.5 (Ref#1 to 4). The calculated extinction coefficient from the sequence (Ref#4) that has been corrected for the large amount of polysaccharide in factor H is 13.2. Despite these differences, which are typical for a widely studied protein, the value of 12.4 is in the middle of the reported range and is closest to the calculated value after correction for the high content of polysaccharide.  

References

Pangburn, M. K., and H. J. Mu¨ller-Eberhard. 1983. Kinetic and thermodynamic analysis of the control of C3b by the complement regulatory proteins factors H and I. Biochemistry 22: 178–185).

Sim, R. B., and R. G. Discipio. 1982. Purification and structural studies on the complement system control protein IH (factor H). Biochem. J. 205: 285–293.

Nagaki, K., K. Iida, M. Okubo, and S. Inai. 1978. Reaction mechanims of 1H globulin. Int. Arch. Allergy Appl. Immunol. 57: 221–232.

Hakobyan, S., C. L. Harris, A. Tortajada, J. E. Goicochea de, A. Garcia-Layana, P. Fernandez-Robredo, C. S. Rodriguez de, and B. P. Morgan. 2008. Measurement of factor H variants in plasma using variant-specific monoclonal antibodies: application to assessing risk of age-related macular degeneration. Invest. Ophthalmol. Vis. Sci. 49: 1983–1990.

譯文：

問：您好，請(qǐng)問A137的消光系數(shù)是如何確定的？是通過實(shí)驗(yàn)測(cè)定的，還是通過其他方法得出的？該數(shù)值與根據(jù)序列計(jì)算的理論消光系數(shù)存在較大差異，謝謝。

答：你好，簡(jiǎn)：

我們采用分光光度法，以12.4的280nm摩爾消光系數(shù)（1%溶液，參考文獻(xiàn)1）測(cè)定了補(bǔ)體因子H（FH）的濃度。MK·潘伯恩博士對(duì)補(bǔ)體因子H有深入的研究，根據(jù)其論文中的解釋，現(xiàn)總結(jié)如下：已有文獻(xiàn)報(bào)道的補(bǔ)體因子H消光系數(shù)范圍為9.0至19.5（參考文獻(xiàn)1至4）；通過氨基酸序列計(jì)算，并針對(duì)補(bǔ)體因子H中大量的多糖成分進(jìn)行校正后，得到的消光系數(shù)為13.2（參考文獻(xiàn)4）。對(duì)于一種被廣泛研究的蛋白質(zhì)而言，出現(xiàn)上述消光系數(shù)的差異是正常現(xiàn)象，而12.4這一數(shù)值處于已報(bào)道的范圍中間，且與經(jīng)多糖高含量校正后的計(jì)算值最為接近。

參考文獻(xiàn)

潘伯恩·M·K、米勒-埃伯哈德·H·J，1983年，補(bǔ)體調(diào)節(jié)蛋白H因子和I因子調(diào)控C3b的動(dòng)力學(xué)與熱力學(xué)分析，《生物化學(xué)》，22卷：178-185頁

西姆·R·B、迪西皮奧·R·G，1982年，補(bǔ)體系統(tǒng)調(diào)節(jié)蛋白IH（H因子）的純化與結(jié)構(gòu)研究，《生物化學(xué)雜志》，205卷：285-293頁

長脅·K、飯?zhí)铩、大久保·M、稻井·S，1978年，1H球蛋白的反應(yīng)機(jī)制，《國際過敏反應(yīng)與應(yīng)用免疫學(xué)文獻(xiàn)》，57卷：221-232頁

哈科布揚(yáng)·S、哈里斯·C·L、托爾塔哈達(dá)·A等，2008年，利用變異型特異性單克隆抗體檢測(cè)血漿中補(bǔ)體因子H變異體及其在年齡相關(guān)性黃斑變性風(fēng)險(xiǎn)評(píng)估中的應(yīng)用，《眼科研究與視光科學(xué)》，49卷：1983-1990頁

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